Journal: Molecular cell

Article Title: RPA phosphorylation inhibits DNA resection

doi: 10.1016/j.molcel.2019.05.005

Figure Lengend Snippet: Key Resources Table

Article Snippet: DNA2 was further purified using a 1 mL HiTrap SP (GE Healthcare, 17115101) with a gradient from buffer C to buffer D (25 mM Tris pH 8.0, 1 M NaCl, 10% Glycerol, 1 mM DTT) and dialyzed overnight at 4°C in Buffer C. Purified human RPA was phosphorylated in vitro as described previously with a few minor modifications ( Fotedar and Roberts, 1992 ; Stillman and Gluzman, 1985 ).

Techniques: Produced, Affinity Purification, Recombinant, Mutagenesis, Expressing, Lambda DNA Preparation, Plasmid Preparation, Software, Microscopy, Avidin-Biotin Assay, Flow Cytometry, Real-time Polymerase Chain Reaction, Imaging

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Expression of stromal-derived factor-1 is decreased by IL-1 and TNF and in dermal wound healing.

doi: 10.4049/jimmunol.166.9.5749

Figure Lengend Snippet: FIGURE 3. Recombinant IL-1a and TNF-a potently inhibit SDF-1 pro- duction by human fibroblasts while increasing the expression of IL-8 and MCP-1. A and B, Primary cultures of human gingival fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of SDF-1 in culture supernatants was measured by ELISA (A) and by a Nalm-6 chemotaxis bioassay (B). p, p , 0.05; pp, p , 0.01 (compared with untreated controls). C–E, Human gingival fibroblasts were stimulated with IL-1a (1 mg/ml) for the indicated period, and levels of mRNA encoding SDF-1a, SDF-1b, IL-8, MCP-1, and GAPDH were determined by RNase protection assay (C). The intensities of the mRNA for GAPDH, SDF-1a, and SDF-1b (D) or IL-8 and MCP-1 (E) were measured, and values for GAPDH were used to normalize for differences in the total amount of RNA analyzed at the indicated time points. F, Primary cultures of human dermal fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of IL-8 in culture supernatants was measured by ELISA. Data in A, B, and F are presented as the mean of three independent experiments that assayed three different fibroblast donors. Error bars exhibit the SD of the values. Data in C, D, and E are from three independent experiments.

Article Snippet: Next, 100 ml of a 1 mg/ml solution of biotinylated, goat anti-human SDF-1b polyclonal Ab (R&D Systems) was added.

Techniques: Recombinant, Expressing, Enzyme-linked Immunosorbent Assay, Chemotaxis Assay, Bioassay, Rnase Protection Assay

Journal: Veterinary Research

Article Title: Increased annexin A1 and A2 levels in bronchoalveolar lavage fluid are associated with resistance to respiratory disease in beef calves

doi: 10.1186/1297-9716-44-24

Figure Lengend Snippet: Mass Spectrometry data for protein spots from DIGE analysis of BALF sampled within 2–3 days of arrival to the feedlot, for which spot densities were greater in calves that remained healthy compared to those that later developed pneumonia

Article Snippet: For detection of annexin A1, the membrane was incubated for 1 h with goat anti-human annexin A1 antibody (1:200; NBP1-50411, Novus Biologicals, Oakville, ON, Canada), then for 30 min with peroxidase-conjugated rabbit anti-goat secondary antibody (1:2000; DakoCytomation, Glostrup, Denmark), and bands were detected by chemiluminescence (ECL Plus, GE Healthcare) visualized with a ChemiDoc system (BioRad, Mississauga, Ontario, Canada).

Techniques: Mass Spectrometry

Journal: Veterinary Research

Article Title: Increased annexin A1 and A2 levels in bronchoalveolar lavage fluid are associated with resistance to respiratory disease in beef calves

doi: 10.1186/1297-9716-44-24

Figure Lengend Snippet: Western blot analysis of BALF samples labeled with antibody to annexin A1. Lane 1: synthetic annexin A1 peptide (positive control), 2: molecular weight ladder, 3: blank, 4: negative control, 5–7: samples from calves that remained healthy, 8–10: samples from calves that later developed pneumonia. The three bands indicate that there are three isoforms of the annexin A1 present in BALF, and the density of the bands is higher in the calves that remained healthy compared to the calves that later developed pneumonia.

Article Snippet: For detection of annexin A1, the membrane was incubated for 1 h with goat anti-human annexin A1 antibody (1:200; NBP1-50411, Novus Biologicals, Oakville, ON, Canada), then for 30 min with peroxidase-conjugated rabbit anti-goat secondary antibody (1:2000; DakoCytomation, Glostrup, Denmark), and bands were detected by chemiluminescence (ECL Plus, GE Healthcare) visualized with a ChemiDoc system (BioRad, Mississauga, Ontario, Canada).

Techniques: Western Blot, Labeling, Positive Control, Molecular Weight, Negative Control

Journal: Veterinary Research

Article Title: Increased annexin A1 and A2 levels in bronchoalveolar lavage fluid are associated with resistance to respiratory disease in beef calves

doi: 10.1186/1297-9716-44-24

Figure Lengend Snippet: Western blot of BALF samples labeled with antibody to annexin A2. Lane 1: synthetic annexin A1 peptide, 2: synthetic annexin A2 peptide (positive control), 3: molecular weight ladder, 4–6: samples from calves that remained healthy; 7–9: samples from calves that later developed pneumonia, 10: negative control. The two bands indicate that there are two isoforms of the annexin A2 present in BALF, and the density of the bands is higher in the calves that remained healthy compared to the calves that later developed pneumonia.

Article Snippet: For detection of annexin A1, the membrane was incubated for 1 h with goat anti-human annexin A1 antibody (1:200; NBP1-50411, Novus Biologicals, Oakville, ON, Canada), then for 30 min with peroxidase-conjugated rabbit anti-goat secondary antibody (1:2000; DakoCytomation, Glostrup, Denmark), and bands were detected by chemiluminescence (ECL Plus, GE Healthcare) visualized with a ChemiDoc system (BioRad, Mississauga, Ontario, Canada).

Techniques: Western Blot, Labeling, Positive Control, Molecular Weight, Negative Control

Journal: Veterinary Research

Article Title: Increased annexin A1 and A2 levels in bronchoalveolar lavage fluid are associated with resistance to respiratory disease in beef calves

doi: 10.1186/1297-9716-44-24

Figure Lengend Snippet: Mass spectrometry data for protein spot 1231

Article Snippet: For detection of annexin A1, the membrane was incubated for 1 h with goat anti-human annexin A1 antibody (1:200; NBP1-50411, Novus Biologicals, Oakville, ON, Canada), then for 30 min with peroxidase-conjugated rabbit anti-goat secondary antibody (1:2000; DakoCytomation, Glostrup, Denmark), and bands were detected by chemiluminescence (ECL Plus, GE Healthcare) visualized with a ChemiDoc system (BioRad, Mississauga, Ontario, Canada).

Techniques: Mass Spectrometry

Journal: Biology

Article Title: Sustained Functioning Impairments and Oxidative Stress with Neurobehavioral Dysfunction Associated with Oral Nicotine Exposure in the Brain of a Murine Model of Ehrlich Ascites Carcinoma: Modifying the Antioxidant Role of Chlorella vulgaris

doi: 10.3390/biology11020279

Figure Lengend Snippet: Representative photomicrograph of the cerebral tissue sections showing the immunoexpression of Bcl-2 (red arrowheads) in the control ( A ), CV ( B ), nicotine-treated ( C ), and nicotine + CV -treated ( D ) and the immunoexpression of the caspase 3 (red arrowheads) in the in the control ( E ), CV ( F ), nicotine-treated ( G ), and nicotine + CV -treated mice ( H ). Scale bar (30 μm).

Article Snippet: For each animal, two consecutive 5 μm thick cerebral tissue sections were prepared; the first was stained for Bcl-2 and the second was stained for caspase-3 antigens using the rabbit polyclonal anti-Bcl-2 primary antibody (ab196495) at a 1/100 dilution, the rabbit monoclonal recombinant anti-caspase-3 primary antibody [EPR18297] (ab184787) at 1/1000 dilution (Abcam Inc.), and the 3,3′-Diaminobenzidine (DAB) chromogen and hematoxylin counterstain following the avidin-biotin-peroxidase complex immunohistochemical technique [ ].

Techniques:

Journal: Biology

Article Title: Sustained Functioning Impairments and Oxidative Stress with Neurobehavioral Dysfunction Associated with Oral Nicotine Exposure in the Brain of a Murine Model of Ehrlich Ascites Carcinoma: Modifying the Antioxidant Role of Chlorella vulgaris

doi: 10.3390/biology11020279

Figure Lengend Snippet: Representative photomicrograph of the cerebral tissue sections showing the immunoexpression of Bcl-2 (red arrowheads) in the control ( A ), CV ( B ), nicotine-treated ( C ), and nicotine + CV -treated ( D ) and the immunoexpression of the caspase 3 (red arrowheads) in the in the control ( E ), CV ( F ), nicotine-treated ( G ), and nicotine + CV -treated mice ( H ). Scale bar (30 μm).

Article Snippet: For each animal, two consecutive 5 μm thick cerebral tissue sections were prepared; the first was stained for Bcl-2 and the second was stained for caspase-3 antigens using the rabbit polyclonal anti-Bcl-2 primary antibody (ab196495) at a 1/100 dilution, the rabbit monoclonal recombinant anti-caspase-3 primary antibody [EPR18297] (ab184787) at 1/1000 dilution (Abcam Inc.), and the 3,3′-Diaminobenzidine (DAB) chromogen and hematoxylin counterstain following the avidin-biotin-peroxidase complex immunohistochemical technique [ ].

Techniques:

Journal: Biology

Article Title: Sustained Functioning Impairments and Oxidative Stress with Neurobehavioral Dysfunction Associated with Oral Nicotine Exposure in the Brain of a Murine Model of Ehrlich Ascites Carcinoma: Modifying the Antioxidant Role of Chlorella vulgaris

doi: 10.3390/biology11020279

Figure Lengend Snippet: Effect of the oral administration of nicotine (100 µg/mL/kg bw), CV (100 mg/kg bw), or both on the concentration of Bcl-2, Caspase 3 immunoexpression, and encephalopathy lesion scoring of female Swiss mice brain.

Article Snippet: For each animal, two consecutive 5 μm thick cerebral tissue sections were prepared; the first was stained for Bcl-2 and the second was stained for caspase-3 antigens using the rabbit polyclonal anti-Bcl-2 primary antibody (ab196495) at a 1/100 dilution, the rabbit monoclonal recombinant anti-caspase-3 primary antibody [EPR18297] (ab184787) at 1/1000 dilution (Abcam Inc.), and the 3,3′-Diaminobenzidine (DAB) chromogen and hematoxylin counterstain following the avidin-biotin-peroxidase complex immunohistochemical technique [ ].

Techniques: Concentration Assay